rip1 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc rip1
Rip1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 662 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rip1/RIP+XP+Rabbit+mAb/pm41817888-65-19-22
Average 96 stars, based on 662 article reviews

rip1 - by Bioz Stars, 2026-11

96/100 stars

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Saline:

Article Title: Study on the Function and Mechanism of Neutrophil Extracellular Traps in Regulating Necroptosis Following Traumatic Brain Injury
Article Snippet: .. The membranes were blocked with 5% skimmed milk prepared in TBST (Tris‐buffered saline with 0.1% Tween‐20) at room temperature for 1 h. They were then incubated overnight at 4°C with the following primary antibodies: PAD4 (1:1000, 214810, Abcam), MPO (1:1000, ab208670, Abcam), Bcl‐2 (1:1000, A0208, Abclonal), Bax (1:1000, A19684, Abclonal), RIP1 (1:1000, #3493, Cell Signaling Technology), RIP3 (1:1000, #95702, Cell Signaling Technology), MLKL (1:1000, #37705, Cell Signaling Technology), P‐RIP1 (1:1000, #31122, Cell Signaling Technology), P‐RIP3 (1:1000, #91702, Cell Signaling Technology), P‐MLKL (1:1000, #37333, Cell Signaling Technology), and GAPDH (1:1000, AB‐P‐R001, GOODHERE Biotech). .. Following the primary antibody incubation, membranes were treated with an HRP‐conjugated secondary antibody (GB23303, Servicebio, 1:10,000) for 1 h at room temperature.

Incubation:

Article Title: Sequestosome-1/p62 Mediates TLR4-Induced Inflammatory Program in Dendritic Cells Under Normoxic and Hypoxic Conditions.
Article Snippet: Equal amounts of total proteins for each sample were separated by 4-42 20% Mini-protean TGX gels (BioRad, Hercules, CA, USA) and transferred onto a 43 nitrocellulose membrane (BioRad, Hercules, CA, USA). .. Membranes were blocked and 44 incubated overnight, at 4°C, with the following primary antibodies: MyD88 (Cell Signaling 45 Technologies,Danvers, MA, 1:1000, Cat.n° 4283); RIP1 (Cell Signaling 46 Technologies,Danvers, MA, 1:1000, Cat.n° 3493); SQSTM1/p62 (Cell Signaling 47 Technologies,Danvers, MA, 1:1000, Cat.n° 5114); ph-p65 (NFκB) (Cell Signaling 48 Technologies,Danvers, MA, 1:1000, Cat.n° 3033); pro-IL-1β (Cell Signaling 49 Technologies,Danvers, MA, 1:1000, Cat.n° 12703); NLRP3 (Cell Signaling 50 Technologies,Danvers, MA, 1:1000, Cat.n° 15101); HIF-1α (BD Biosciences, San Jose, 51 CA, USA, 1:200 Cat.n◦ 610958); and b-actin (Sigma-Aldrich, St. Louis, MO, USA, 52 1:50000, Cat.n° A3854). .. After washing, membranes were incubated with horseradish 53 peroxidase (HRP)-conjugated secondary anti-mouse I anti-rabbit antibodies (Cell Signaling 54 Technologies, Danvers, MA, USA).

Article Title: Sequestosome-1/p62 Mediates TLR4-Induced Inflammatory Program in Dendritic Cells Under Normoxic and Hypoxic Conditions
Article Snippet: Equal amounts of total proteins for each sample were separated by 4–20% Mini-protean TGX gels (BioRad, Hercules, CA, USA) and transferred onto a nitrocellulose membrane (BioRad, Hercules, CA, USA). .. Membranes were blocked and incubated overnight, at 4 °C, with the following primary antibodies: MyD88 (Cell Signaling Technologies,Danvers, MA, 1:1000, Cat.n° 4283); RIP1 (Cell Signaling Technologies,Danvers, MA, 1:1000, Cat.n° 3493); SQSTM1/p62 (Cell Signaling Technologies,Danvers, MA, 1:1000, Cat.n° 5114); ph-p65 (NFκB) (Cell Signaling Technologies,Danvers, MA, 1:1000, Cat.n° 3033); pro-IL-1β (Cell Signaling Technologies,Danvers, MA, 1:1000, Cat.n° 12703); NLRP3 (Cell Signaling Technologies,Danvers, MA, 1:1000, Cat.n° 15101); HIF-1α (BD Biosciences, San Jose, CA, USA, 1:200 Cat.n◦ 610958); and b-actin (Sigma-Aldrich, St. Louis, MO, USA, 1:50000, Cat.n° A3854). .. After washing, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary anti-mouse I anti-rabbit antibodies (Cell Signaling Technologies, Danvers, MA, USA).

Article Title: RIP1 Exacerbates BBB Disruption by Impairing Autophagy-Mediated A2 Astrocyte Polarization in Hypertension-Induced Cerebral Microhemorrhage in Mice.
Article Snippet: have been identified as major risk factors contributing to the development of CMHs [2].. The accumulation of multiple CMHs can progressively impair neuronal function and lead to cognitive decline [3, 4].. An animal study further revealed that aging and hypertension interact synergistically to exacerbate the formation of CMHs [5].

Western Blot:

Article Title: Mebendazole induces ZBP-1 mediated PANoptosis of acute myeloid leukemia cells by targeting TUBA1A and exerts antileukemia effect
Article Snippet: Necrostatin-1 (T 1847), Necrosulfonamide (T 6904) and Z-VAD(OMe)-FMK (T 6013) were obtained from TargetMol. .. Primary antibodies for western blot used were as follows: p-MLKL (ab187091, Abcam), MLKL (#14993, CST), p-RIP3 (#93654, CST), RIP3 (#13526, CST), p-RIP1 (#65746, CST), RIP1 (#3493, CST), cleaved caspase-1 (#4199, CST), cleaved caspase-3 (#9661, CST), NLRP3 (#15101, CST), TUBA1A (ab95966, Abcam), GAPDH (AF0006, Beyotime), and β-actin (ACTB) (AF0003, Beyotime). .. Antibodies for flow cytometry used were as follows: human CD45-FITC (304006, Biolegend, USA), CD11b-APC (301310, Biolegend) and CD11c-FITC (337214, Biolegend).

Cell-Signaling:

Article Title: The C3–C3aR axis drives rotenone-induced cognitive damage via synaptic engulfment, dark microglia and PANoptosis
Article Snippet: The C3aR inhibitor (SB290157) was purchased from Selleck (SB290157, Shanghai, China). .. Antibodies were obtained from various sources as follows: C3 (ab200999), Neu-N (ab177487), Fibrinogen (ab92572), ser129-phosphorylated α-synuclein (ab51253), and phosphorylated-RIP3 (ab95117) from Abcam (Waltham, MA, UK); C3aR and Iba-1 antibodies from Santa Cruz Biotechnology (sc-133172, Dallas, TX, USA) and Wako Chemicals (019–19741, Tokyo, Japan), respectively; GFAP (16825), Bcl2 (68103), and Bax (50599) antibodies from Proteintech (Wuhan, Hubei, China); PSD95 (3450S), ERK (9101S), p-ERK (4695T), p65 (8242T), p-p65 (3033S), IκBα (2859T), p-IκBα (4814T), RIP1 (53286S), p-RIP1 (3493S), MLKL (37333S), p-MLKL (37705S), and RIP3 (95702S) antibodies from Cell Signaling Technology (Danvers, MA, USA). .. The C3a ELISA kit was purchased from CUSABIO (CSB- E08511 m, Houston, TX, USA), and TUNEL assay kit was provided by KeyGEN (KGA7073-1, Nanjing, China.

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